Their chain lengths, including the amino alcohol, were 11, 14, and 18 residues, respectively. By using the well-established, HPLC/MS-based peptaibiomics approach, it could unequivocally be demonstrated that all of these formulations contained new and recurrent peptaibols, i.e., peptaibiotics carrying an acetylated N-terminus, the C-terminus of which is reduced to a 1,2-amino alcohol. We analyzed five commercial biocontrol agents (BCAs), namely Canna((R)), Trichosan((R)), Vitalin((R)), Promot((R)) WP, and TrichoMax((R)), formulated with recently described species of the Trichoderma harzianum complex, viz. This feature is of major practical importance, as the detection of peptaibiotics in plant-protective Trichoderma species, which are successfully used against economically relevant bacterial and fungal plant pathogens, certainly contributes to a better understanding of these complex antagonistic interactions. The production of bioactive polypeptides (peptaibiotics) in vivo is a sophisticated adaptation strategy of both mycoparasitic and saprotrophic Trichoderma species for colonizing and defending their natural habitats.
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